Why should there be no bubbles in the slide?

Question

We have this experiment called "Preparing a Temporary WET Mount", and so what we did, we put a leaf in a glass slide and put some drops of water. Then cover it with a cover slip. Our teacher said, there should be no bubbles in the slide. WHY?

Answer 1

Bubbles will change the refractive index of the medium and cause distortion of the image

So you wont get a clear picture in the microscope

It would be the same as having water spray on your sunglasses - you would get a blurred picture