Question:

Why should we store the restriction enzyme at -20ºC?

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anyone can tell me the reason why?

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2 ANSWERS


  1. It prevents denaturation, aka enzyme degradation. Heat kills protein linkages.  


  2. Not just restriction enzymes are supposed to be stored at freezing temperatures, many other enzymes indicate that they're required to be kept at -20ºC, like the DNA polymerase.

    Putting the enzymes at freezing temperatures helps to lower the levels of energy that surrounds it, which equals to a diminished level of movement (because you know that proteins are moving, fluid entities), and by doing so, you help to protect their tri-dimentional structure, which is the one thing that enable the proteins to be named enzymes

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