Why we need to autoclave the media in media preparation?describe principles of sterilization by autoclaving.?

Question

why we need to autoclave the media in media preparation?describe principles of sterilization by autoclaving.

Answer 1

to kill unwanted organism,their spores etc so that we can assure that only what we inoculated grow...

sterilization can be achieved by heating at 121deg for 15 min or by dry method by incubating in 180deg for 1hour.

Answer 2

There are bacteria everywhere, even in the distilled water you use to prepare the medium. To study any bacterium, the first thing you have to do is to remove any contamination.

Media can be sterilized at 121 C for 15 minutes in an autoclave. That temperature can kill all endospores.